Phage display is a method to identify functional peptide motifs by exploiting phage biology and bacterial metabolism. In order to construct a library information on the phage coat proteins, we can insert randomized DNA sequences into specific locations of the phage genome. What is the minimum number of random DNA sequences we need to insert to construct more than a million of library peptide motifs? Describe why.
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(a) Phage display is a method to identify functional peptide motifs by exploiting phage biology and bacterial metabolism. In order to construct a library information on the phage coat proteins, we can insert randomized DNA sequences into specific locations of the phage genome. What is the minimum number of random DNA sequences we need to insert to construct more than a million of library peptide motifs? Describe why.
(b) Based on the assumption that we can construct 100% unbiased 4mer peptide library through randomized gene insertion into phage genome, what is the probability to have the specific binding peptides for the TNT recognition (WHWQ) from the phage display screening process?
Phage display is a method to identify functional peptide motifs by exploiting phage biology and bacterial metabolism. In order to construct a library information on the phage coat proteins, we can insert randomized DNA sequences into specific locations of the phage genome. What is the minimum number of random DNA sequences we need to insert to construct more than a million of library peptide motifs? Describe why.
Phage display is a method to identify functional peptide motifs by exploiting phage biology and bacterial metabolism. In order to construct a library information on the phage coat proteins, we can insert randomized DNA sequences into specific locations of the phage genome. What is the minimum number of random DNA sequences we need to insert to construct more than a million of library peptide motifs? Describe why.
Phage display is a method to identify functional peptide motifs by exploiting phage biology and bacterial metabolism. In order to construct a library information on the phage coat proteins, we can insert randomized DNA sequences into specific locations of the phage genome. What is the minimum number of random DNA sequences we need to insert to construct more than a million of library peptide motifs? Describe why.
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