In bacteria, researchers have isolated strains that carry mutations within tRNA genes. These mutations can change the sequence of the anticodon. For example, a normal tRNAlrpgene would encode a tRNA with the anticodon 3′-ACC-5′. A mutation could change this sequence to 3′-CCC-5′. When this mutation occurs, the tRNA still carries a tryptophan at its 3′ acceptor stem, even though the anticodon sequence has been altered.
A. How would this mutation affect the synthesis of polypeptides within the bacterium?
B.What does this mutation tell you about the recognition between tryptophanyl-tRNA synthetase and tRNATrp? Does the enzyme primarily recognize the anticodon or not?
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