From an experimental viewpoint, what does it mean to say that an RFLP is associated with a trait? Let’s suppose that two strains of pea plants differ in two RFLPs that are linked to two genes governing pea size. RFLP-1 is found in 2000-bp and 2700-bp bands, and RFLP-2 is found in 3000-bp and 4000-bp bands. The plants producing large peas have RFLP-1 (2000 bp) and RFLP-2 (3000 bp); those producing small peas have RFLP-1 (2700 bp) and RFLP-2 (4000 bp). A cross is made between these two strains, and the F( offspring are allowed to self-fertilize. Five phenotypic classes are observed: small peas, small-medium peas, medium peas, medium-large peas, and large peas. We assume that each of the two genes makes an equal contribution to pea size and that the genetic variance is additive. Draw a gel and explain what RFLP banding patterns you would expect to observe for these five phenotypic categories. Note: Certain phenotypic categories may have more than one possible banding pattern.
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