A get retardation assay can be used to determine if a protein bids to a segment of DNA. When a segment of DNA is bound by a protein. Its mobility will be retarded, and the DNA band will appear higher in the gel. In the gel retardation assay shown here, a cloned gene fragment that is 750 bp in length contains a regulatory element that is recognized by a trascripion factor called protein X. Previous experiments have shown that the presence of hormone X result in transcriptional activation by protein X. The results of a gel retardation assay are shown here.
A get retardation assay can be used to determine if
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Get Help Now!Explain the action of homone X.
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